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Published Application Notes

Measure cell proliferation and cell growth independently using automated cell count, mitosis detection and cell dry mass accumulation.

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Automatically measure single-cell random motility parameters from live cell populations in formats up to 96-well plates with the Livecyte Kinetic Cytometer.

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Automatically separate the effects of cell proliferation, experimental setup and types of cellular motion on the outcome of a scratch wound assay. The Livecyte Kinetic Cytometer produces single-cell motility parameters for all cells surrounding the wound, giving additional insights as to why a wound has closed faster.

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Assessing macrophage M1 activation in response to pro-inflammatory mediators leading to macrophage morphology, growth and proliferative changes.

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Quantifying the phagocytosis of E. coli bioparticles by RAW 264.7 macrophages down to the single-cell level.

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Assay quantifying the efferocytosis of apoptotic Jurkat cells by RAW 264.7 macrophages. By analysing down to the single-cell level we are able to provide a much more complete picture of macrophage phenotypic behaviour than previously possible in such an assay.

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Quantifying the effect of different treatments on cell mitotic behaviour, from number of mitosis event, to mitotic time.

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Effects of far-red fluorescent labelling and LED irradiation on cell behaviour during long-term time-lapse microscopy using a Livecyte Kinetic Cytometer.

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Quantify changes in yeast strain morphology and growth with Livecyte

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Go beyond traditional wound healing assays with the Phasefocus Livecyte.

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Use non-invasive time-lapse imaging to quantify cell death without labels, dyes or phytotoxic damage.

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Produce a Staurosporine dose response curve without use of fluorescent labels.

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Analyse and extract morphological and dynamic phenotypes to identify heterogeneity within mixed cancer cell populations.

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A case study to demonstrate how analysis of a population to an individual cell level can reveal subtle differences in cell behaviour that would be unresolved by a population averaged approach.

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