High content live cell assays from 96 wells to single cells
A label-free high-content system
Livecyte changes what's possible to measure using live cell assays
Livecyte produces exceptionally high contrast time-lapse videos using Phasefocus’s patented Ptychographic quantitive phase imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence.
Automated single-cell tracking of even the most sensitive cells quickly reveals subtle phenotypic differences in unperturbed cell populations.
Easy-to-use Dashboards present coherent and concise results from up to 96 wells at a time whilst retaining the ability to investigate individual cell behaviour and outlying characteristics.
From Images to Insights
Easy-to-use image processing and tracking algorithms allow individual cells to be identified in real time.
Explore how the properties of those identified cells, and how they change over time.
Powerful Integrated Analysis
Integrated analysis suite enables cell behaviour to be monitored as a function of treatment
Easily export results in Excel and Graphpad formats
Monitor many different types of behaviour, from morphological to motile, in every experiment making laboratory time more productive
Large fields of view (up to 4mm2) in an assay relevant plate format (up to 96 well plate) mean the behaviour of many cells can be captured making results statistically robust.
Unlike other QPI systems, Livecyte's ptychography technology removes the need for plate inserts or expensive consumables - reducing running costs compared to comparative systems.
Correlate changes in proliferation, motility and morphology as a function of treatment, from every experiment.
Example outputs from a motility dose response assay
Explore Population Heterogeneity
Identify, track and classify individuals and monitor their phenotypic behaviour over time.
Classify subpopulations based of any combination of parameters.
Compare subpopulation behaviour as a function of treatment.
Mitotic cells identified from their high thickness/low area phenotype
Subpopulation of cells highlighted based on mCherry expression.
Automatically segment and track individuals and identify their division events and children.
Quick and easy track correction enables perfect lineage determination.
Explore single cell or whole lineage phenotypical properties across multiple generations.
A comparison of the growth rates of two lineages within the same population
|Imaging Modalities||Brightfield; Ptychographic quantitative phase imaging; Fluorescence (up to 7 channels)|
|Compatible Plate Formats||Standard 6, 12, 24, 48, 96 well plates (glass and high-grade plastic), 35mm dishes, slides (inc. Ibidi µ-slides)|
|Required Plate Inserts||None|
|Objective Lens Options||4x; two from 10x, 20x or 40x (Plan N, Fluorite and UPLXAPO options available, air only)|
|Camera||High sensitivity sCMOS (2048 x 2048 pixels; 6.5µm pixel size; 16 bit; 82% Peak QE)|
|XY Stage||Automatic Linear-encoded ASI stage|
|Z Stage||Automatic Olympus Z-positioner|
|Ptychographic QPI Illumination||Integrated low-power laser diode (typically 30µW/mm2 at 650nm wavelength)|
|Fluorescence Illumination||CoolLED pE-300 or CoolLED pE-4000 options available|
|Fluorescence Filters||System compatible with up to 7 channels (in addition to QPI modality)|
|QPI Field of View||Up to 2 x 2mm with 10x lens|
|Lateral Resolution||Up to 0.4µm with a UPLXAPO 20x|
|QPI Capture Rate||Up to one image per 13s|
|Environment Control||Integrated stand-alone incubator controlling temperature, CO2, humidity and evaporation|
|IT||Bespoke Acquisition PC, Analysis PC and Network Attached Storage (up to 40Tb) options|
|Software||Acquire acquisition software, Cell Analysis Toolbox analysis software available|
|Space Requirements||1800 x 800 x 900 mm (W x D x H)|
|Weight||~65kg depending on configuration|
|Power Requirements||Nominal 700 W|
|Gas Requirements||Supply of 5% CO2 (in air) either from a cylinder with regulator or from mains supply via a CO2 mixer|