Home > Applications > Wound Healing

Scratch Wound Assays on Livecyte

 

Livecyte uses high contrast images to automatically segment and track single cells in up to 96 well plate format. Combined with its large fields of view (up to 4mm²) this enables users to...

  • Overcome the limitations of confluence-based approaches by directly measuring cell motility
  • Automatically determine leading edge cell speed and directionality
  • Definitively separate out migration from proliferative or morphological behaviours using Analyse's Dashboards.

See an example assay in the Application Note

 

 

Directly Measure Cell Motility

 

Single cell segmentation and speed information

Livecyte can measure the migration of single cells in wound healing assays rather than overly simplistic confluence-based models.

 

It does this by automatically tracking individual cells and directly measuring their motility over time.

 

This allows determination of both speed and direction of motion, giving a richer description of how a wound closes.

 

Mouse over the image on the right to show the trails coloured by cell speed.

 

 

Leading Edge Cell Determination

 

Scratch wound with leading edge cells highlighted

Livecyte can automatically identify and track leading edge cells.

 

The speed and directness of leading edge cells can be compared to the rest of the population and explicitly between different regions and treatments.

 

 

Watch the Webinar...

 

Scratch Wound

 

Interest in finding out more?

Hear Martin talk through how Livecyte could help you over come the limitations of confluence-based wound healing assays.

 

 

 

Watch it here...

 

 

 There is nothing out there that can do what the Livecyte does.
There are huge array of analysis possibilities that allow you to ask questions that no other system can answer.  

 

Dr Mat Hardman, University of Hull