Home > Applications > Cell Proliferation and Growth

Live Cell Proliferation Assays

 

Livecyte's quantitative phase imaging modality enables users to obtain fluorescence-like high contrast images, without the need for fluorescence labels or phototoxic illumination, in plate formats up to 96 wells.

Additionally Livecyte measures cell dry mass - a unique QPI property that independently quantifies cell growth from cell division events.

Automatic single-cell segmentation and tracking identifies individual cells and enables the following of cell proliferation and growth for long periods of time, at both the assay and single-cell levels.

See an example assay in the Application Note

 

 

Livecyte Measures True Cell Growth

 

Confluence, used by other live cell analysis systems, is an imperfect measure of cell growth.

  • Cells can spread out.
  • Cells can contract or ball-up.
  • Cells can divide symmetrically or asymmetrically.
  • Cells can grow without dividing.

Also you can differentiate between all these different behaviours with Livecyte's cell dry mass measurement.


Dry mass is a measure of the amount of matter in each cell, providing a direct measurement of cell growth.

 

 

Compare Growth at the Single Cell Level

 

This video shows two cell lineages within a cancer cell population highlighted in Livecyte's Analyse software.

 

The dry mass plot (top right) shows the cells growing and dividing.  The green cell grows faster than the orange cell - both cell divide at the same dry mass (size), but the green cell lineage consistently proliferates faster than the orange cell throughout its lineage.

 

 

Watch the Webinar...

 

Cell Growth & Proliferation

 

Interested in finding out more?

Hear Martin talk through how Livecyte's ability to independently measure Cell Proliferation and Growth could help your research in this short webinar.

 

 

Watch it here...

 

 

 There is nothing out there that can do what the Livecyte does.
There are huge array of analysis possibilities that allow you to ask questions that no other system can answer.  

 

Dr Mat Hardman, University of Hull